Green fluorescent protein
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The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.
- See also: Wikipedia
- Related: pGLO, Yellow fluorescent protein
| A comprehensive article on fluorescent proteins at... A comprehensive article on fluorescent proteins at Scholarpedia www.scholarpedia.org/article/Fluorescent_proteins - Web |
| Brief summary of landmark GFP papers Brief summary of landmark GFP papers www.nature.com/nmeth/focus/fluorescence/classics/proteins.html - Web |
| Video of 2008 Nobel Prize lecture of Roger Tsien o... Video of 2008 Nobel Prize lecture of Roger Tsien on fluorescent proteins nobelprize.org/mediaplayer/index.php?id=1070 - Web |
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| Excitation and emission spectra for various fluore... Excitation and emission spectra for various fluorescent proteins tsienlab.ucsd.edu/Documents/REF%20-%20Fluorophore%20Spectra.xls - Web |
| Green Fluorescent Protein Chem Soc Rev themed issu... Green Fluorescent Protein Chem Soc Rev themed issue www.rsc.org/.../Article.asp?Type=Issue&JournalCode=CS&Issue=10&Volume=38&SubYear=2009 - Web |
