SDS-PAGE
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SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry, forensics, genetics and molecular biology to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins. In most proteins, the binding of SDS to the polypeptide chain imparts an even distribution of charge per unit mass, thereby resulting in a fractionation by approximate size during electrophoresis.
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- Related: Capillary electrophoresis, DNA electrophoresis, Eastern blotting, Electroblotting, Electrophoresis, Gel electrophoresis, History of electrophoresis, Isoelectric focusing, Isotachophoresis, Native Gel Electrophoresis, Northern blot, Protein electrophoresis, Southern blot, Two dimensional SDS-PAGE, Western blot, Zymography
| Demystifying SDS-PAGE Video Demystifying SDS-PAGE Video sdspage.homestead.com/TheVideo.html - Web |
| Demystifying SDS-PAGE Demystifying SDS-PAGE sdspage.homestead.com - Web |
| SDS-PAGE Calculator SDS-PAGE Calculator www.omx-online.com/calculator.html - Web |
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| 2-Dimensional Protein Gelelectrophoresis 2-Dimensional Protein Gelelectrophoresis wwwzb.zb.kfa-juelich.de/.../index.php?title=Sample_preparation_2D - Web |
