Complementary DNA

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Output from a cDNA microarray used in testing

In genetics, complementary DNA (cDNA) is DNA synthesized from a messenger RNA (mRNA) template in a reaction catalyzed by the enzyme reverse transcriptase and the enzyme DNA polymerase. cDNA is often used to clone eukaryotic genes in prokaryotes. When scientists want to express a specific protein in a cell that does not normally express that protein (i.e., heterologous expression), they will transfer the cDNA that codes for the protein to the recipient cell. cDNA is also produced by retroviruses (such as HIV-1, HIV-2, Simian Immunodeficiency Virus, etc.) which is integrated into its host's genome where it creates a provirus.

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H-Invitational Database H-Invitational Database www.h-invitational.jp - Web

Functional Annotation of the Mouse database Functional Annotation of the Mouse database fantom.gsc.riken.jp - Web

cDNA Animation cDNA Animation www.maxanim.com/genetics/cDNA/cDNA.htm - Web

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Complementary DNA tool Complementary DNA tool www.bugaco.com/calculators/dna_reverse_complement.php - Web

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